Raman spectroscopy and optical trapping are emerging tools in regenerative medicine which allow to discriminate, classify and eventually sort the different amniotic cell types in a label-free and non-invasive manner. Therefore, we want to establish a data base of the Raman molecular signatures of fetal and adult fibroblasts, keratinocytes, chondrocytes and endothelial cells as a sort of reference cell populations. This will allow to study the bio-chemical changes of skin and cartilage during development. Subsequently, we want to categorize the heterogeneous composition of the amniotic fluid cell pool into clusters and compare them with the data base. This will allow to discriminate and classify and eventually sort the different amniotic cell types in a label-free and non-invasive manner as alternative to FACS and MACS.
